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. Author manuscript; available in PMC: 2019 Dec 24.
Published in final edited form as: Nat Med. 2019 Jun 24;25(7):1164–1174. doi: 10.1038/s41591-019-0461-z

Extended Data Fig. 10. Treg cell-specific deletion of Rorc and Myd88 impairs mucosal tolerance.

Extended Data Fig. 10.

(a-d) Analysis of sIgA+ (a,b) and IgE+ (c.d) fecal bacteria in OVA/SEB-sensitized Foxp3YFPCre, Il4raF709Foxp3YFPCre and Foxp3YFPCreRorcΔ/Δ mice. Fecal pellets of Rag2−/− and Igh7−/−Il4raF709 mice were used as negative controls [Foxp3YFPCre (n=6 per group), Il4raF709Foxp3YFPCre (n=11 and 7), and Foxp3YFPCreRorcΔ/Δ mice (n=10 and 8)]. (e-f) Analysis of GATA3+Foxp3+ Treg cells in the following OVA/SEB-sensitized mice that were either untreated or treated with Clostridiales or Bacteroidales consortia: Il4raF709Foxp3YFPCre (n=9, 5 and 5), and Il4raF709Foxp3YFPCreRorcΔ/Δ (n= 4, 5 and 8). (g,h) Analysis of GATA3+Foxp3+ Treg cells in OVA/SEB-sensitized Il4raF709Foxp3YFPCre mice treated with the Bacteroidales consortium (n=9), and in OVA/SEB-sensitized Il4raF709Foxp3YFPCreMyd88Δ/Δ mice otherwise untreated or treated with the Clostridiales or Bacteroidales consortia (n= 8, 9 and 8). Each symbol represents one mouse. Results represent Means ± S.E.M. collated from 2 independent experiments. P values were derived by one-way ANOVA with Dunnett post hoc analysis (b,d,f,h).