Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Escherichia coli) | E. coli T7 Express ΔclpA ΔclpP ΔclpX | this paper | E. coli strain lacking the ClpA, ClpP, and ClpX genes. progenitor: E. coli T7 Express (New England Biolabs #C2566) | |
| Recombinant DNA reagent | pT7 ClpXΔN(plasmid) | Martin et al., 2005 | N-terminally His6-tagged ClpXΔN (residues 62–424) for overexpression | |
| Recombinant DNA reagent | pT7 ClpP (plasmid) | Kim et al., 2000 | C-terminally His6-tagged ClpP for overexpression | |
| Recombinant DNA reagent | pBAD ClpP/ClpXΔN(plasmid) | this paper | for inducible polycistronic expression of ClpP and ClpXΔN(residues 62–424) for cytoplasmic GFP degradation assays. Progenitor: pBAD (Guzman et al., 1995; jb.177.14.4121–4130.199) | |
| Recombinant DNA reagent | pBAD null (plasmid) | this paper | control plasmid for cytoplasmic GFP degradation assays. Progenitor: pBAD (Guzman et al., 1995) | |
| Recombinant DNA reagent | ProD GFP Gly12 ssrA (plasmid) | this paper | for constitutive expression of GFP (residues 1–229) substrates with a 12xGly cassette and partial ssrA (GSENYALAA). All other substrates are derivatives of this construct with different variable cassette sequences. Progenitor: ProD Gemini (Davis et al., 2011; nar/gkq81) | |
| Recombinant DNA reagent | pT7 GFP Gly12 ssrA (plasmid) | this paper | for overexpression of N-terminally His6-tagged GFP (1-229) substrates with a 12xGly cassette and partial ssrA (GSENYALAA). All other substrates are derivatives of this construct with different variable cassette sequences. |