Fig. 6.

Tau expression was increased by oligo Aβ-42via Sirt3 modulation. Primary cortical neurons from newborn hTau mice were transfected with vector, Sirt3 shRNA (knockdown), or Sirt3 cDNA (overexpression). a Neurons were shown being transfected with the vector (green). About 90% neurons were successfully transfected. The transfected neurons were then treated with oligo Aβ−42 (1000 ng/ml) for 24 h. b A representative Western blot of total tau (multiple bands ranging from 50 to 64 kDa), Ac-tau, and Sirt3 proteins was shown. c Total tau, d Ac-tau, and e Sirt3 levels were normalized with β-actin, an internal control. N = 6 per group; *p< 0.05 and **p< 0.01