Figure 2.

EC359 decrease cell viability, colony formation, invasion and promote apoptosis of TNBC cells that express LIF and LIFR. The expression of LIF and LIFR in TNBC (A), ER+ BC and normal mammary epithelial cells (B) was determined by western blotting. Effect of increasing doses of EC359 on the cell viability of TNBC (C) ER+BC and normal mammary epithelial cells (D) was determined using the MTT cell viability assay (n=3). E, Effect of inducible CRISPR/Cas9 mediated KO of LIFR on EC359 induced cell viability was determined using MTT assays in BT-549 cells (n=3). F, Effect of EC359 (20 nM) on cell survival was measured using colony formation assays. G, Effect of EC359 (25 nM) on cell invasion of MDA-MB-231 and BT-549 model cells was determined using matrigel invasion chamber assays (n=3). Representative images of invaded cells are shown and the number of invaded cells in five random fields was quantitated. H, Effect of indicated doses of EC359 on caspase 3/7 activity (Caspase-Glo3/7 assay) and I, Annexin V staining in MDA-MB-231 and BT-549 cells (n=3) was determined. ** P<0.01, ****p<0.0001.