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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cancer Res. 2019 Jul 17;79(15):3983–3991. doi: 10.1158/0008-5472.CAN-19-0717

Fig. 2. FLPo-mediated recombination in GFAP-positive neural stem cells.

Fig. 2.

(A) Immunostaining analysis of GFAP (green) and tdTomato (red) expression in the subgranular zone (SGZ) of the hippocampal dendate gyrus (DG) of 3, 8 and 35-week-old GFAP-FLPo; CAG-FRT-stop-FRT-tdTomato animal. Scale bars, 20 μm. Arrows mark GFAP-negative/ tdTomato positive cells.

(B) Immunostaining analysis of NeuN (green) and tdTomato (red) expression in the SGZ of the hippocampal DG of 3, 8 and 35-week-old GFAP-FLPo; CAG-FRT-stop-FRT-tdTomato animal. Scale bars, 20 μm. Arrows mark NeuN-positive/ tdTomato positive cells.

(C) Quantified analysis of GFAP-positive and NeuN-positive cells in the SGZ of the hippocampal DG of 3, 8 and 35-week-old GFAP-FLPo; CAG-FRT-stop-FRT-tdTomato animal. Data are means with SEM. 3 animals per condition were subjected to the analysis.

(D) Immunostaining analysis of NeuN (green) and tdTomato (red) expression in the cortex of 35-week-old GFAP-FLPo; CAG-FRT-stop-FRT-tdTomato animal. Scale bars, 20 μm.