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. 2019 Aug 2;2:283. doi: 10.1038/s42003-019-0539-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The solo β4 LTR sequence interrupts agouti gene expression in dorsal but not ventral skin. a Schematic of homologous recombination from nonagouti (a) to black-and-tan (a^t) or β4(direct-rec) alleles. b Representative appearance of the β4(direct-rec) mutant. Heterozygous β4(direct-rec) (a/β4(direct-rec)) mice exhibited yellow color in ventral but not dorsal hairs. c RT-PCR of the agouti transcripts in dorsal and ventral skins of neonatal β4(direct-rec) (a/β4(direct-rec)), and β4-del (a/β4-del) mice. The agouti transcripts were amplified from exons 1A, 1A′, 1B, and 1C to 3. Transcript numbers are shown on the right. The agouti transcripts from exons 2 to 3 were also amplified. β-actin was used as an internal control. Asterisks indicate not analyzed. Wild-type agouti (C3H; A/A), wild-type white-bellied agouti (MSM; A^mol/A^mol), nonagouti (B6; a/a), and nonagouti with piebald (JF1; a/a) mice are shown as positive or negative controls. D, dorsal skin; V, ventral skin. d Agouti transcript structures detected via RT-PCR analyses. Transcript numbers are shown beside the splicing connections. The splicing patterns of dorsal and ventral skins are shown separately for each β4(direct-rec) and nonagouti (a) allele. Scale bar = 500 bp