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. 2019 Aug 2;9:11240. doi: 10.1038/s41598-019-47682-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo quantification of the nanosensor. (a) Confocal image of SenALiB-676n expressed in the cytosol of S. cerevisiae. (b) Time-course emission intensities analysis of the donor and acceptor fluorophores on addition of As³⁺. (c) The graph indicates FRET ratio change in the cytosol of a single yeast cell and was compared with that of control after addition of 10 µM As³⁺ along with ratiometric images.