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. 2019 Aug 2;9:11277. doi: 10.1038/s41598-019-47653-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Type VII collagen expression in corneal development. Expression initially appeared in the corneal epithelium (CE) of E14 (blue arrow), with no expression associating with the surface ectoderm (SE) at E12. By E16 type VII collagen (blue arrow) appeared localised along the epithelium basement membrane. The positive control of an adult mouse cornea (data not presented) showed the usual type VII collagen expression along the basement membrane of the corneal epithelium. No expression was present in the no primary antibody control and the rabbit IgG control images (data not shown). Blue (DAPI – cell nucleus), red (type VII collagen). Scale bars = 50 μm.