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. 2019 Aug 2;9:11277. doi: 10.1038/s41598-019-47653-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopy imaging showed high resolution images of the developing cornea at E10. High resolution images of the neural crest cells that had migrated into the area of the developing cornea showed a large quantity of organelles and vesicles intracellularly and extracellularly (black arrows). (A) Synthesising organelles were also seen within the mesenchymal cells, including mitochondria, endoplasmic reticulum and Golgi apparatus (blue arrows). (B) Extracellular material (red arrows) extended from the cells of the surface ectoderm (SE) into the developing corneal stroma (C).