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. 2019 Aug 2;9:11277. doi: 10.1038/s41598-019-47653-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopy of the developing corneal stroma at E14 showed high resolution images of the collagen fibrils. (A,B) At lower magnification, the collagen fibrils were seen to be abundant, surrounding the cells of the corneal stroma. (A) The collagen fibrils appeared to have started to cluster within bundles, with the bundles arranged orthogonally between cells. (A) An abundance of cell projections was seen within the corneal stroma, with some associating with the collagen fibrils (A, red arrows). The collagen fibrils were imaged at a higher magnification to show the orthogonal arrangement more clearly (B).