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. 2019 Aug 2;9:11277. doi: 10.1038/s41598-019-47653-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The developing eye at E15 was imaged at a low magnification (×540) and images stitched together to show the lens (L), retina (R), corneal stroma (C) and corneal epithelium (E). (A) Reconstructions at a high magnification within the area of the black box were made in the anterior corneal stroma (AS) following the method described in Fig. 1. (A,B) The reconstructions showed sub-epithelial cells to have flattened, now being similar to the cell morphology in the posterior cornea. The datasets were segmented using volren and showed extensive projections from the corneal stroma cells connecting to adjacent cells (Fig. C, black arrows). To see the reconstructions in more detail, please refer to Supplementary Video 5.