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. 2019 Aug 2;10:3484. doi: 10.1038/s41467-019-11476-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Bioactive GA biosynthesis during early embryogenesis. a qRT-PCR analysis of NtGA3ox1, NtGA3ox2 and NtGA3ox3 expression; GAPDH was used as the internal control. b Expression of genes related to GA biosynthesis [NtGA20ox1 (Ntc12, AB012856.1) and NtGA20ox2 (Ntc16, AB016084.1)], deactivation [NtGA2ox1 (AB125232.1), NtGA2ox1-like (XM_016586923.1), NtGA2ox3 (EF471117), and NtGA2ox5 (EF471118)], and signaling in WT two-celled proembryos (stage 1) via transcriptome analysis, and via qRT-PCR analysis in WT four-celled proembryos (stage 2). GAPDH was used as the internal control. Values are means ± SE of three biological replicates. nd, not detected. c Expression of NtGA3ox1 and NtGA3ox3 during embryogenesis. d Expression of NtGA3ox2 and NtGA3ox3 in the developing seed coat. Asterisks, micropylar ends; Scale bars: 10 μm (c), 100 μm (d). The source data of the graphs are provided in the Source Data file