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. 2019 Aug 2;10:3469. doi: 10.1038/s41467-019-11409-0

Fig. 5.

Fig. 5

Physiological properties of HDAC4/5-deficient excitatory neurons. Intrinsic excitability and synaptic strength of CA1 pyramidal cells were assessed by electrophysiological whole-cell recordings from acute brain slices from p20 mice. a Sample traces of depolarization-induced action potentials monitored in current-clamp mode. b Averaged numbers of action potentials, plotted as a function of stimulus intensity. Control: n = 5 mice/18 neurons; DKO: n = 3/27. c Sample traces of spontaneous miniature excitatory postsynaptic currents (sEPSCs) recorded in voltage-clamp mode. Holding potentials were −70 mV. d Averaged sEPSC amplitudes and frequencies. Control: n = 5 mice/20 neurons; DKO: n = 5/26. e Traces of evoked AMPA (inward) and NMDA (outward) excitatory postsynaptic currents (eEPSCs) monitored at −70 and +40 mV holding potentials, respectively, in the presence of the GABA receptor blocker, Picrotoxin (50 μM). Synaptic responses were triggered by electrical stimulation of CA3 afferents in the Schaffer collateral path. f Averaged AMPA eEPSC amplitudes (left) and AMPA/NMDA ratios (right). Control: n = 3 mice/11 neurons; DKO: n = 3/11. g Sample traces of AMPA eEPSC elicited by repetitive stimulation at 10 Hz. h Averaged eEPSC amplitudes during 10 Hz trains, normalized to amplitudes of first responses. Control: n = 5 mice/22 neurons; DKO: n = 5/22. All quantifications are represented as Mean ± SEM. *p < 0.05 (t-test)