Figure 6.
The RHD of TonEBP Is Required for MMS-Induced TonEBP Focus Formation, PCNA Polyubiquitination, and Cell Survival in Response to MMS Treatment
(A) The deleted portion of the TonEBP RHD in the TonEBPΔ allele is marked on the homodimer of TonEBP (PDB: 1IMH).
(B) MEFs from TonEBPΔ/Δ (ΔDBD) mice and their wild-type (WT) littermates were treated with 0.01% (v/v) MMS. TonEBP in the nucleus was visualized by immunostaining. Representative images of the nucleus are shown in each condition. Scale bar, 2 μm.
(C) MEFs were treated with 0.01% (v/v) MMS, and chromatin-bound PCNA was detected as described in Figure 3A.
(D) MEFs were treated with MMS at various doses as indicated, and live cells were counted; mean ± SD, n = 5, *p < 0.05.
(E and F) ΔDBD MEFs were transfected with the indicated siRNAs. The cells were immunoblotted (E), and cell survival after MMS treatment was analyzed (F); mean ± SD, n = 3.