Table 1.

Primer/probe sets used for 16s rDNA PCR.

Primer/probe	Sequence
Fusobacterium spp.
forward	5′ GGA TTT ATT GGG CGT AAA GC 3′
reverse	5′ GGC ATT CCT ACA AAT ATC TAC GAA 3′
probe	FAM 5′CTC TAC ACT TGT AGT TCC G 3′ TAMRA
Group B Streptococci
forward	5′ ATC CTG AGA CAA CAC TGA CA 3′
reverse	5′ TTG CTG GTG TTT CTA TTT TCA 3′
probe	JOE 5′ ATC AGA AGA GTC ATA CTG CCA CTT 3′ TAMRA
Mycoplasma hominis
forward	5′ ATT GAT TGC TGC AGG TGA TAC A 3′
reverse	5′ GGT GTT ACA ATA TCA GCC CCA AC 3′
probe	FAM 5′ AGA GCA GCG GCA GTT GAA 3′ TAMRA
Peptostreptococcus micros
forward	5′ GCC GTA AAC GATGAG TGC TAG G 3′
reverse	5′ CCA GGC GGA ATG CTT AGT GT 3′
probe	FAM 5′ TGG GAG TCA AAT CTC GCC G 3′ TAMRA
Sneathia/Leptotricia
forward	5′ AAT TAT TGG GCT TAA AGG GCA TC 3′
Reverse (S)	5′ CTA CAA AAC TGT ATA ACT AGA GTA CT 3′
Reverse (L)	5′ CTA CAA AAC TGT TGA ACT AGA GTA C 3′
probe	FAM 5′ ACA AGT TGA AGG TGA AAA CCT RTG GC 3′ TAMRA
Ureaplasma parvum
forward	5′CAT TGA TGT TGC ACA AGG AGA AA 3′
reverse	5′ TTA GCA CCA ACA TAA GGA GCT AAA TC 3′
probe	FAM 5′ TTG ACC ACC CTT ACG AG 3′ TAMRA
Ureaplasma urealyticum
forward	5′ ATC GAC GTT GCC CAA GGG GA 3′
reverse	5′ TTA GCA CCA ACA TAA GGA GCT AAA TC 3′
probe	JOE 5′ TTG TCC GCC TTT ACG AG 3′ TAMRA
Human C reactive protein
forward	5′ CTT GAC CAG CCT CTC TCA TGC 3′
Reverse	5′ TGA AGT AGA CCC CAC CC 3′
Probe	JOE 5′ TTT GGC CAG ACA GGT AAG GGG CAC 3′ TAMRA