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. 2019 Aug 2;9:11262. doi: 10.1038/s41598-019-47592-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding and incorporation of ATM-3507 into Tpm3.1/actin filaments. (a) Increasing amounts of ³H-ATM-3507 (0–22 µM) pre-incubated with Tpm3.1 do not affect formation of Tpm-actin filament. Protein fractions of the Tpm-actin from both pellets and supernatants after centrifugation of the Tpm-actin mixture with increasing concentrations of ATM-3507. Full-length gels are presented in Supplementary Fig. 1. (b) The molar ratio of ATM-3507 to the junction formed by the N-terminal and C-terminal ends of Tpm dimers as a function of ATM-3507 concentration; n = 3. (c) Incorporation of ³H-ATM-3507 into Tpm/actin filament under two different conditions of (i) pre-incubated (Pre, black squares) ³H-ATM-3507 with 5 μM Tpm3.1 prior to actin addition, or (ii) post-incubated (Post, gray circles) ³H-ATM-3507 with the Tpm3.1/F-actin filaments; n = 4 for each curve.