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. 2019 Aug 2;10:3480. doi: 10.1038/s41467-019-11471-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Exclusive root apex-specific amyloplast localization in seed plants. a Living representative species from different plant lineages included in the analysis (from left to right): K. flaccidum (green alga), P. patens (moss), S. moellendorffii (lycophyte), C. richardii (fern), P. taeda (gymnosperm), G. arboreum, and A. thaliana (dicots), and O. sativa (monocot). b Lugol’s staining of the rhizoid (P. patens). c–h mPS-PI staining of the root tips from S. moellendorffii (c), C. richardii (d), P. taeda (e), G. arboreum (f), A. thaliana (g), and O. sativa (h). The blue arrows indicate root hair initiation. The yellow arrows indicate the apical cell (QC-like cell) in the fern C. richardii and the QC in seed plants. The dashed red rectangles indicate the zone with amyloplasts. Scale bars, 20 µm