Table 2.
Expression of activation markers CD69, TLR2 and HLA-DR on monocytes 24 hours after protocol “antigens after PF”. After a total incubation time of 24 hours, samples were fixated for flow cytometric analysis of surface marker expression. Displayed are mean values of double-positive staining as a percentage (CD14/CD69, CD14/TLR2, CD14/HLA-DR) in samples from three differe nt blood donors with ± SD.
| antigens after PF | Basal | LPS | HKLM | PWM | |||||
|---|---|---|---|---|---|---|---|---|---|
| CD14+ | mean | SD | mean | SD | mean | SD | mean | SD | |
| CD69+ | ground | 18,56 | ±9,91 | 30,91 | ±17,64 | 13,28 | ±7,47 | 27,20 | ±18,09 |
| inflight | 19,49 | ±8,10 | 31,05 | ±6,82 | 12,01 | ±6,79 | 28,88 | ±13,56 | |
| TLR2+ | ground | 62,93 | ±12,76 | 50,29 | ±12,47 | 35,68 | ±10,66 | 60,73 | ±13,18 |
| inflight | 56,65 | ±18,12 | 48,09 | ±14,82 | 38,56 | ±8,91 | 62,72 | ±7,53 | |
| HLA-DR+ | ground | 50,73 | ±15,77 | 51,12 | ±13,60 | 28,06 | ±1,63 | 53,99 | ±7,49 |
| inflight | 43,30 | ±22,95 | 45,63 | ±19,16 | 28,88 | ±10,18 | 50,39 | ±11,50 | |