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. 2019 Aug 2;9:10648. doi: 10.1038/s41598-019-46128-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rescue-like behaviour test depending on the tube condition. (A) Upper row: a sample image of the ball of yarn. Lower row: a sample image showing the back of the tube containing the ball of yarn with a paper lid. (B) A sample image of an opaque 50-mL tube with a paper lid. (C) Schematic diagram of the test. Rescue-like behaviour test for cage-mates: time spent in the cage-mate area or the empty area. (D) Rescue-like behaviour test for a ball of yarn: time spent in the ball of yarn area or the empty area. (E) Rescue-like behaviour test for the opaque tube: time spent in the opaque tube area or the empty area. (F) Comparison of the latency to lid-opening in the three tests. (G) Comparison of the time spent in each area of the three tests. All data are presented as box plots. Statistical significance is represented as *p < 0.05. n.s., not significant. The p values were calculated by one-way repeated measures ANOVA (F) and paired t-test (G). n = 10 animals per test.