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. 2019 Aug 2;10:3490. doi: 10.1038/s41467-019-11442-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — A ZF1 degron reporter in the ER is protected from ZIF-1/ECS-mediated degradation. a mCherry-tagged SP12 localizes to the endoplasmic reticulum (ER) in anterior (somatic, arrow) and posterior (germ line, arrowhead) cells (n = 102). Scale bar: 10 µm. b An RFP- and ZF1 degron-tagged KDEL reporter similarly localizes to the ER in both anterior (somatic, arrow) and posterior (germ line, arrowhead) cells, despite expression of the ZIF-1 ligase adaptor in anterior cells (n = 88). c There was no significant difference in the ratio between the fluorescence of anterior AB lineage cells and of posterior germ line or germ line sister cells at the indicated stages (p > 0.05, Student’s t-test with Bonferroni correction, n = 18–25 mCh::SP12, n = 24–28 ss::tagRFP-T::ZF1::KDEL). Bars represent mean ± s.e.m. Source data are provided as a Source Data file. d The ER membrane protects luminal proteins from ubiquitination by cytosolic ligase complexes