Fig. 3.

An extrinsic factor from BMSCs is responsible for the impaired mobilization of KO HSPCs from the BM into the periphery. a–d Lethally irradiated WT and KO CD45.2 mice were transplanted with LSK cells from WT CD45.1 mice. n = 5 per group. a Schematic representation of LSK cell adoptive transfer. b Absolute number of donor-derived cells in the BM, thymus (Thy), and spleen (SP) of recipients. c Absolute number of donor-derived T cells, B cells, and myeloid cells (Myeloid) in the spleen of recipients. d Absolute number of donor-derived LSK cells (LSK) and LK cells (LK) in the BM of recipients. e–h Lethally irradiated WT CD45.1 mice were transplanted with LSK cells from WT or KO CD45.2 mice. n = 5 per group. e Schematic representation of LSK cell adoptive transfer. f Absolute numbers of donor-derived cells in the BM, Thy, and SP of recipients. g Absolute numbers of donor-derived T cells, B cells, and myeloid cells in the spleen of recipients. h Absolute numbers of donor-derived LSK and LK cells in the BM of recipients. Statistical significance was assessed by two-tailed Student’s t-test. **P < 0.01. All data are presented as the mean ± SEM. Source data are provided as a Source Data File