Fig. 6.

Phc2 binds to the Vcam1 gene locus and suppresses Vcam1 gene expression through histone modifications. a Schematic representation of the Vcam1 gene locus. The locations of primers #1 to #7 and exons are indicated. b ChIP was performed with WT and KO BM cell lysates using anti-Phc2 Ab (Phc2) or a control Ab (Control). DNA recovered from ChIP was then analyzed by qPCR. n = 5. c ChIP was performed using the same samples with indicated Abs. DNA recovered from ChIP was then analyzed by qPCR. n = 6. Control, ChIP using each isotype control Ab. d, e BMSCs (OP9 cells) were treated with various concentrations of GSK126 for 48 h. qRT-PCR (d) and immunoblot (e) analyses were then performed to measure the VCAM-1 expression levels. n = 3. f BMSCs (OP9 cells) were treated with GSK126 (5 μM) for 48 h. ChIP was performed using cell lysates with indicated Abs. DNA recovered from ChIP was then analyzed by qPCR. n = 5. Control, ChIP using each isotype control Ab. Statistical significance was assessed by two-tailed Student’s t-test. *P < 0.05; **P < 0.01. All data are presented as the mean ± SEM. Source data are provided as a Source Data File