Fig. 7.

Blocking the interaction between VCAM-1 and VLA-4 restores steady-state HSPC mobilization in KO mice. a Relative ratio of migrated WT LSK cells (LSK) through WT or KO BMSCs in the presence of anti-VLA-4 Ab and/or anti-VCAM-1 Ab. n = 5 per group. Statistical significance was assessed by two-tailed Student’s t-test. *P < 0.05; **P < 0.01. b Relative ratio of adhered WT or KO LSK to KO BMSCs in the presence of anti-VLA-4 Ab and/or anti-VCAM-1 Ab. n = 5 per group. Statistical significance was assessed by two-tailed Student’s t-test. *P < 0.05; **P < 0.01. c, d CFSE-labeled LSK cells from WT mice were intravenously injected into lethally irradiated WT or KO mice. Anti-VCAM-1 Ab or its isotype control Ab (2 mg kg⁻¹) was intravenously injected into WT and KO recipient mice 1 h before adoptive cell transfer. n = 5 per group. c The frequencies of CFSE⁺ donor cells were measured in the BM, PB, and, spleen (SP) of recipients at 16 h after adoptive cell transfer. d The frequencies of donor-derived clonogenic progenitors (CFU-C) homing to the BM (femur), PB and SP of recipients at 16 h after adoptive cell transfer. Statistical significance was assessed by one-way ANOVA with Tukey HSD analysis. Mean values not sharing the same superscript letter (^{a, b, c}) differ significantly at P < 0.05. e–g Anti-VCAM-1 Ab or its isotype control Ab (2 mg kg⁻¹) was intravenously injected into WT and KO mice for 3 days. n = 5 per group. e Comparison of WBC counts between WT and KO mice on day 3 after Ab treatment. f Absolute number of splenocytes for WT and KO mice on day 3 after Ab treatment. g CFU-C in the BM (femur), PB, and SP of WT and KO mice on day 3 after Ab treatment. Statistical significance was assessed by two-tailed Student’s t-test. **P < 0.01. All data are presented as the mean ± SEM. WT, WT recipient mice; KO, KO recipient mice. “−“, treatment with an isotype control Ab; “ + “, treatment with anti-VCAM-1 Ab. Source data are provided as a Source Data File