. 2019 Aug 2;10:3496. doi: 10.1038/s41467-019-11386-4

Table 1.

Absolute numbers of immune cells and their precursors in Phc2^+/+ and Phc2^−/− mice^a

Tissue	Population	Surface phenotype	Phc2^+/+	Phc2^−/−	p-value	n
BM (×10⁴)	LSK	Lin⁻Sca-1⁺c-kit⁺	3.68 ± 0.75	3.62 ± 0.52	0.953	5
	LT-HSC	Lin⁻Sca-1⁺c-kit⁺Flt3⁻CD34⁻	0.56 ± 0.12	0.58 ± 0.04	0.813	5
	SLAM-HSC	Lin⁻Sca-1⁺c-kit⁺CD48⁻CD150⁺	0.65 ± 0.25	0.73 ± 0.26	0.869	5
	ST-HSC	Lin⁻Sca-1⁺c-kit⁺Flt3⁻CD34⁺	1.35 ± 0.18	1.50 ± 0.22	0.615	5
	MPP	Lin⁻Sca-1⁺c-kit⁺Flt3⁺CD34⁺	1.53 ± 0.18	1.89 ± 0.42	0.449	5
	CMP	Lin⁻Sca-1⁻c-kit⁺CD16/32⁻CD34⁺	10.57 ± 2.24	10.01 ± 2.23	0.862	5
	GMP	Lin⁻Sca-1⁻c-kit⁺CD16/32⁺CD34⁺	14.25 ± 1.35	14.75 ± 2.59	0.87	5
	MEP	Lin⁻Sca-1⁻c-kit⁺CD16/32⁻CD34⁻	7.72 ± 1.67	6.83 ± 1.05	0.663	5
	CLP	Lin⁻Sca-1⁻c-kit⁺CD127⁺	0.90 ± 0.12	1.01 ± 0.12	0.566	5
	T cell	CD3ε⁺	183.57 ± 40.24	178.34 ± 16.64	0.907	5
	B cell	B220⁺	1317.83 ± 157.03	1357.77 ± 112.11	0.837	5
	Pro/pre B cell	B220⁺IgM⁻IgD⁻	793.78 ± 106.97	752.46 ± 112.88	0.796	6
	Immature B cell	B220⁺IgM⁺IgD⁻	250.34 ± 47.52	245.04 ± 28.29	0.926	6
	Myeloid cell	CD11b⁺	3480.12 ± 466.76	3260.69 ± 368.49	0.722	5
	NK cell	NK1.1⁺	83.95 ± 11.97	93.97 ± 16.35	0.634	5
Thymus (×10⁶)	ETP	Lin⁻CD25⁻CD44⁺c-kit⁺	0.11 ± 0.01	0.03 ± 0.00	<0.001	5
	DN1	CD4⁻CD8⁻CD44⁺CD25⁻	0.35 ± 0.08	0.08 ± 0.01	0.011	5
	DN2	CD4⁻CD8⁻CD44⁺CD25⁺	0.15 ± 0.04	0.04 ± 0.00	0.036	5
	DN3	CD4⁻CD8⁻CD44⁻CD25⁺	1.55 ± 0.40	0.44 ± 0.09	0.027	5
	DN4	CD4⁻CD8⁻CD44⁻CD25⁻	2.35 ± 0.71	0.43 ± 0.09	0.027	5
	DP	CD4⁺CD8⁺	66.42 ± 4.50	17.69 ± 2.49	<0.001	5
	CD4 SP	CD4⁺CD8⁻	10.45 ± 1.28	2.72 ± 0.51	0.001	5
	CD8 SP	CD4⁻CD8⁺	4.94 ± 0.46	1.41 ± 0.38	<0.001	5
Spleen (×10⁶)	T cell	CD3ε⁺	20.72 ± 1.91	7.72 ± 0.73	<0.001	5
	CD4 + T cell	CD4⁺	12.18 ± 0.99	4.59 ± 0.38	<0.001	5
	CD8 + T cell	CD8⁺	8.12 ± 0.15	3.21 ± 0.34	<0.001	5
	B cell	B220⁺	40.24 ± 2.81	15.89 ± 1.13	<0.001	5
	Immature B cell	B220⁺IgM⁺IgD⁻	8.52 ± 0.66	3.85 ± 0.31	<0.001	6
	Mature B cell	B220⁺IgM⁺IgD⁺	24.93 ± 0.75	11.67 ± 1.12	<0.001	6
	Macrophage	CD11b⁺F4/80⁺	4.89 ± 1.03	1.75 ± 0.35	0.021	5
	Dendritic cell	CD11b⁺CD11c⁺	2.55 ± 0.67	0.83 ± 0.17	0.038	5
	NK cell	NK1.1⁺	0.14 ± 0.02	0.05 ± 0.01	0.003	5

LSK Lin⁻Sca-1⁺c-kit⁺ cell, LT-HSC long term-hematopoietic stem cell, SLAM-HSC Lin⁻CD41⁻CD48⁻CD150⁺cell, ST-HSC short term-hematopoietic stem cell, MPP multipotent progenitor, CMP common myeloid progenitor, GMP granulocyte-monocyte progenitor, MEP megakaryocyte-erythroid progenitor, CLP common lymphoid progenitor; ETP early T-cell precursor, DN double negative cell, SP single positive cell

Source data are provided as a Source Data File

^ap-values were generated by two-tailed unpaired Student’s t-test. All data represent the mean ± SEM