Table 1.
Isothermal titration calorimetry (ITC) measurements of NHE1ct with CN
| NHE1cta | KD (nM) | ΔH (kcal mol−1) | TΔS (kcal mol−1) |
|---|---|---|---|
| NHE1ct (NHE1 I680-Q815) | |||
| NHE1ct WTb | 59 ± 9 | −12 ± 2 | −2.5 ± 1.4 |
| NHE1ct motif variants | |||
| NHE1ctAVATAA | 2400 ± 500 | −7.6 ± 0.7 | 0.9 ± 0.2 |
| NHE1ctATAP | 4800 ± 500 | −4.5 ± 0.7 | −2.8 ± 0.8 |
| NHE1 motif peptides | |||
| PxIxIT (NHE1 E709-P727) | 6500 ± 500 | −2.9 ± 1.4 | 4.2 ± 1.5 |
| LxVP (NHE1 Q678-P694) | 4300 ± 500 | −8.6 ± 1.0 | −1.3 ± 1.1 |
| Minimal NHE1 CN-binding domain | |||
| NHE1ctΔ92 (NHE1ct I680-S723) | 21 ± 2 | −8.5 ± 0.4 | 2.0 ± 0.3 |
| NHE1ctΔ92 (PVIVIT) | 4.0 ± 0.4 | −9 ± 2 | 2.7 ± 2.3 |
| Charge neutral NHE1ct dynamic linker | |||
| NHE1ctΔ92RARA | 21 ± 2 | −12.2 ± 0.1 | −1.7 ± 1.2 |
| TxxP mutant | |||
| NHE1ct P782A | 48 ± 2 | −2.8 ± 0.1 | 7.2 ± 0.1 |
All measurement with n = 2 or 3
aWT NHE1ct and all NHE1ct variant measurements performed with WT CN (CNA1–370:CNB1–170)
bErrors (±) are S.D.