Table 1.
Newly sequenced Clostridium tetani strains of this study and previously sequenced strains, their originand sequencing data.
| Strain | Clade | Genome (Mbp) | Sequencing coverage | Contigs | N50 (in kb) | CDSs* | Plasmid (kp) | TeNT | Origin, strain collection** | Year | Geographical origin |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Harvard | 1A | 2.839 | 176 | 39 | 263 | 2,810 | 73.5 (single contig) | yes | PC | 1949 | North America |
| Strain 3 | 1A | 2.839 | 254 | 39 | 264 | 2,807 | ca. 73.3 | yes | PC | 1955 | Denmark |
| 4784 A | 1A | 2.836 | 323 | 45 | 155 | 2,809 | ca. 72.7 | yes | PC | 1968 | |
| 1586-U1 | 1A | 2.808 | 381 | 42 | 199 | 2,734 | ca. 72.7 | yes | Sanofi | 1969 | France |
| 1586-Z1 | 1A | 2.735 | 632 | 42 | 199 | 2,654 | no | no | Sanofi | 1969 | France |
| 641-84 | 1A | 2.813 | 277 | 34 | 271 | 2,749 | ca. 72.4 | yes | human, NRC | 1984 | France |
| 46-1-08 | 1A | 2.889 | 194 | 50 | 158 | 2,834 | ca. 72.4 | yes | human, NRC | 2008 | France |
| 407-86 | 1A | 2.810 | 326 | 44 | 222 | 2,786 | no | no | human, NRC | 1986 | France |
| 75-97 | 1A | 2.896 | 236 | 45 | 222 | 2,864 | ca. 72.5 | yes | human, NRC | 1997 | France |
| 89-12 | 1A | 2.900 | 462 | 41 | 200 | 2,870 | ca. 72.8 | yes | NRC, human (tumefaction) | 2012 | France |
| TMB2 | 1B | 2.807 | 473 | 30 | 319 | 2,777 | 69.3 (single contig) | yes | human, PC | 1956 | France |
| B4 | 1 C | 2.818 | 279 | 40 | 248 | 2,757 | ca. 61.7 | yes | PC | 1962 | |
| 1240 | 1 C | 2.804 | 211 | 51 | 201 | 2,744 | ca. 59.9 | yes | cat wound, PC | 1955 | France |
| 1337 | 1 C | 2.817 | 236 | 44 | 271 | 2,755 | ca. 61.6 | yes | PC | 1955 | France |
| 3582 | 1D | 2.945 | 399 | 42 | 230 | 2,868 | ca. 91.1 | yes | human wound, PC | 1964 | France |
| COR1 | 1E | 2.860 | 267 | 48 | 235 | 2,845 | ca. 78.2 | no | Human (uterus perforation), PC | 1955 | France |
| 329 | 1E | 2.861 | 243 | 50 | 235 | 2,850 | ca. 67.9 | no | PC | 1965 | URSS |
| 157-15 | 1E | 2.762 | 453 | 40 | 215 | 2,683 | ca. 52.6 | no | human (M 30 y), femur fracture NRC | 2014 | France |
| 512-15 | 1F | 2.866 | 132 | 52 | 130 | 2,781 | ca. 69.3 | yes | cheese, PC | 1955 | Vietnam |
| 358-99 | 1F | 2.811 | 207 | 56 | 130 | 2,726 | ca. 53.7 | yes | human, NRC | 1999 | France |
| 202-15 | 1F | 2.816 | 407 | 68 | 146 | 2,743 | ca. 67.5 | yes | human (F 49 y), fracture/osteomyelitis, NRC | 2015 | France |
| 132CV | 1G | 2.808 | 122 | 63 | 147 | 2,723 | 63.2 (single contig) | yes | PC | 1955 | Germany |
| 2017-061 | 1H | 2.838 | 238 | 42 | 271 | 2,770 | ca. 58.8 | yes | Human (M 54 y), fracture/septic knee arthritis, NRC | 2016 | France |
| 3483 | 2 | 2.937 | 413 | 66 | 131 | 2,949 | 75.3 (single contig) | yes | human tetanus, PC | 1964 | France |
| 63-05 | 2 | 2.951 | 243 | 59 | 121 | 2,960 | ca. 75.8 | yes | human (F 84 y), wound tetanus, NRC | 2005 | France |
| 778-17 | 2 | 2.878 | 289 | 32 | 237 | 2,799 | ca. 76.7 | yes | human wound, NRC | 2017 | France |
*predicted by RAST.
**PC, Prevot’s Collection; NRC, National Reference Center for Anaerobic bacteria and Botulism, France.
M, male; F, female; y, year.