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. 2019 Aug 2;10:3499. doi: 10.1038/s41467-019-11447-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — GLCC1-induced cell survival depends on glycolytic metabolism. a, b Extracellular acid ratio (ECAR) upon cells were measured after transfecting with control or GLCC1 siRNA1/2 in DLD-1 (a) and HT29 (b). OM, oligomycin; 2-DG, 2-deoxyglucose. c, d The relative lactic acid level was detected in DLD-1 (c) and HT29 (d) cells transfected with control or GLCC1 siRNA1/2. e, f The change of ECAR level with different treatment in LoVo (e) and SW480 (f) cells was measured after transfecting with control or GLCC1 overexpression plasmid. g, h The relative lactic acid level in LoVo (g) and SW480 (h) cells was examined transfected with control or GLCC1 overexpression plasmid. i, j Cell proliferation assay was performed in LoVo and SW480 cells transfected with control plasmid, GLCC1 overexpression plasmid, 2-DG, 2-DG and GLCC1 overexpression plasmid (n = 6). Data are presented as the mean ± SE. p-values were calculated by one-way ANOVA followed by SNK multiple comparison test. *p < 0.05