Fig. 9.

Knockdown of Gal3 ameliorates HD symptoms in R6/2 mice. Mice of 6 weeks were intrastriatally injected with the shLgals3-expressing or shGFP-expressing lentivirus, and monitored for an additional 7 weeks (n = 9–11 mice in each group). a The motor functions of R6/2 mice and their littermate controls were assessed by rotarod performance. b Kaplan–Meier plots of R6/2 and WT mice (P = 0.0089). c Body weight of the indicated animal was measured from 7 to 13 weeks of age. Brain tissues were carefully removed and subjected to immunofluorescence staining to determine the amount of mHTT aggregates (green, EM48; d, e) or DARPP32 (green, a marker for the medium spiny neurons (MSNs), g, h), on the neurons (NeuN, red; a neuronal marker; d, f, g). Nuclei were stained with Hoechst (blue). Four animals were analyzed in each group. The results were analyzed by two-way ANOVA followed by Tukey’s post hoc test. Data are presented as the means ± SEM. *Specific comparison between WT and R6/2 mice infected with the same lentivirus; #Specific comparison between mice of the same genotype. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Same P-value denotation for #. Scale bar: 10 µm. Source data is available as a Source Data File