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. 2019 Aug 1;85(16):e00579-19. doi: 10.1128/AEM.00579-19

FIG 3.

FIG 3

Homologous and heterologous DIP competition in insect feeding assays with FAW, Spodoptera frugiperda. DIP competition dose-response assays were performed by using a panel of native proteins, indicated by the symbols at the top, and the following competitor proteins: Cry1Da_7-DIP (A) Cry1B.868-DIP (B), Cry1F.842-DIP (C), Vip3A-DIP (D), Cry1A.105 (E), and Cry2Ab-DIP (F). The absolute DIP doses used are indicated on the x axis for each of the competition series, and the following fixed concentrations of NIPs were used: 690 ng/cm2 Cry1Da_7 (A to F), 5,520 ng/cm2 Cry1B.868 (A to F), 20.7 ng/cm2 Cry1F.842 (A to D), 2,760 ng/cm2 Vip3A (A to D), 690 ng/cm2 Cry1A.105 (E and F), and 2,760 ng/cm2 Cry2Ab2 (E and F). Statistical analyses of the data were done using multiple comparisons after ordinary one-way ANOVA and a Tukey post hoc test (α = 0.05); the symbols above the bars indicate these results with reference to buffer treatment (negative control), whereas symbols above the connector lines inform about differences between the connected treatment groups (ns, nonsignificant [P > 0.05]; *, P < 0.05; **, P < 0.01; ***, P < 0.001).