TABLE 1.
Comparative assessment of insecticidal proteins in insect feeding assays using susceptible and Cry1F-resistant fall armyworms, Spodoptera frugiperda
| Sample | Dosea (ng/cm2) | S. frugiperda colonyb | Total no. of insect larvae | No. of larvae/repeat | Phenotypic distribution (%) (mean ± SD)c |
|||
|---|---|---|---|---|---|---|---|---|
| Dead | 1st instar | 2nd instar | 3rd instar | |||||
| Buffer | 0 | SS | 40 | 8 | 5.1 ± 11.1 | 0 ± 0 | 0 ± 0 | 94.9 ± 16.8 |
| 0 | Cry1Fa-RR | 40 | 8 | 2.5 ± 5.6 | 0 ± 0 | 7.5 ± 6.8 | 90 ± 5.6 | |
| Cry1Da_7 | 690 | SS | 40 | 8 | 87.5 ± 12.5 | 12.5 ± 12.5 | 0 ± 0 | 0 ± 0 |
| 690 | Cry1Fa-RR | 40 | 8 | 100 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| Cry1Da_7 | 6,900 | SS | 40 | 8 | 97.5 ± 5.6 | 2.5 ± 5.6 | 0 ± 0 | 0 ± 0 |
| 6,900 | Cry1Fa-RR | 40 | 8 | 97.5 ± 5.6 | 2.5 ± 5.6 | 0 ± 0 | 0 ± 0 | |
| Cry1B.868 | 690 | SS | 40 | 8 | 97.5 ± 5.6 | 2.5 ± 5.6 | 0 ± 0 | 0 ± 0 |
| 690 | Cry1Fa-RR | 40 | 8 | 80.0 ± 6.8 | 20.0 ± 6.8 | 0 ± 0 | 0 ± 0 | |
| Cry1B.868 | 6,900 | SS | 40 | 8 | 97.5 ± 5.6 | 2.5 ± 5.6 | 0 ± 0 | 0 ± 0 |
| 6,900 | Cry1Fa-RR | 40 | 8 | 87.5 ± 12.5 | 12.5 ± 12.5 | 0 ± 0 | 0 ± 0 | |
| Cry1F.842 | 690 | SS | 40 | 8 | 90.0 ± 10.0 | 10.0 ± 10.0 | 0 ± 0 | 0 ± 0 |
| 690 | Cry1Fa-RR | 40 | 8 | 0 ± 0 | 0 ± 0 | 2.5 ± 5.6 | 97.5 ± 5.6 | |
| Cry1F.842 | 6,900 | SS | 40 | 8 | 97.5 ± 5.6 | 2.5 ± 5.6 | 0 ± 0 | 0 ± 0 |
| 6,900 | Cry1Fa-RR | 39 | 8 | 0 ± 0 | 0 ± 0 | 5.1 ± 7.0 | 94.9 ± 7.0 | |
a
Amount of protein sample per surface area of diet in a diet overlay assay.
b
SS, Cry1Fa-susceptible insect colony; Cry1Fa-RR, Cry1Fa-resistant insect colony.
c
Average phenotypic distribution of larvae across repeats.