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Verification of the excision of modules 1, 2, and 3 by Flp recombinase. (A) Principle of the PCR verification of the Flp-catalyzed excision of modules 1 to 3 (PCR 3; Fig. 2A shows PCR 3 on nonexcised pOSV802). (B) PCR fragments obtained by PCR 3; expected sizes, 4,192 bp for M145/pOSV802 and 1,637 bp for M145 containing pOSV802 after excision of modules 1 to 3 by the Flp recombinase.
