TABLE 3.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pCR-Blunt | E. coli cloning vector | Invitrogen |
| pRT801 | Source of the φBT1 integrase fragment | 45 |
| pAC-BETA | Source of the origin of replication p15A | 48 |
| pOSV408 | Source of the origin of transfer | 46 |
| pSET152 | Source of the apramycin resistance cassette and of the φC31 integrase fragment | 47 |
| psB1C3-BBa_K1155003 | Source of the amilCP cassette | iGEM registry of standard biological parts |
| pKT02 | Source of the VWB integrase fragment | 52 |
| pOSV215 | Source of the T4 terminator | 54 |
| pOSV554 | Source of the integrase pSAM2 fragment | Our unpublished data |
| pOSV400 | Source of the ORF of hygromycin resistance gene | Our unpublished data |
| pOSV401 | Source of the ORF of kanamycin resistance gene | Our unpublished data |
| pSL128 | Source of the albonoursin cluster (albA, albB, and albC) | 35 |
| pCEA001 | pUC57 containing rpsl(TP)p and tipA RBS | Genecust |
| pCEA002 | pGEM-T easy containing rpsl(TP)p and tipA RBS with the last 6 nucleotides replaced by the SpeI site | This work |
| pCEA003 | Plasmid pCR-Blunt containing pSAM2 integrase, used for site-directed mutagenesis | This work |
| pCEA004 | Plasmid pCR-Blunt containing VWB integrase, used for site-directed mutagenesis | This work |
| pCEA005 | pOSV802 containing rpsl(TP)p and tipA RBS with the last 6 nucleotides replaced by the SpeI site | This work |
| pCEA006 | pOSV802 containing the genes albA, albB, and albC instead of the amilCP cassette | This work |
| pCEA007 | pOSV802 containing rpsl(TP)p and the albonoursin cluster instead of amilCP | This work |
| pOSV800 | Plasmid constructed containing apramycin resistance and φBT1 integrase with two BioBrick sites NheI and SpeI in φBT1 integrase | This work |
| pOSV801 | Plasmid constructed containing apramycin resistance and φBT1 integrase | This work |
| pOSV802 | Plasmid constructed containing apramycin resistance and φC31 integrase | This work |
| pOSV803 | Plasmid constructed containing apramycin resistance and pSAM2 integrase | This work |
| pOSV804 | Plasmid constructed containing apramycin resistance and VWB integrase | This work |
| pOSV805 | Plasmid constructed containing hygromycin resistance and φBT1 integrase | This work |
| pOSV806 | Plasmid constructed containing hygromycin resistance and φC31 integrase | This work |
| pOSV807 | Plasmid constructed containing hygromycin resistance and pSAM2 integrase | This work |
| pOSV808 | Plasmid constructed containing hygromycin resistance and VWB integrase | This work |
| pOSV809 | Plasmid constructed containing kanamycin resistance and φBT1 integrase | This work |
| pOSV810 | Plasmid constructed containing kanamycin resistance and φC31 integrase | This work |
| pOSV811 | Plasmid constructed containing kanamycin resistance and pSAM2 integrase | This work |
| pOSV812 | Plasmid constructed containing kanamycin resistance and VWB integrase | This work |
| pCAS008 | pOSV812 with cassette SP22p-cgc22-T4 terminator instead of amilCP | This work |