. 2019 Aug 2;9:11261. doi: 10.1038/s41598-019-47650-2

Table 2.

Oligonucleotides.

Name	Sequence (5′-3′)	Use
Operonlac-PS1	ATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGTAGGCTGGAGATGCTTC	Amplification of Km^r gene from pKD4
Operonlac-PS2	TAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATCATATGAATAT	Amplification of Km^r gene from pKD4
MG1655-opvA	ATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATTCATTTGGTTATAAATAGAG	Amplification of the opvAB operon (wild type and variants)
MG1655-opvB	TAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATGAGTTTATCTCTGCGCAATGT
pCE40lacY-gfp-5	GCTTTCCCTGCTGCGTCGTCAGGTGAATGAAGTCGCTTAATAAGAAGGAGATATACATATGAG	Amplification of gfp and Cm^r genes from pZEP07
pCE40-km-3	AAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTTTATCACTTATTACAGGCGTA	Amplification of gfp and Cm^r genes from pZEP07
opvA-aac6-F	TCTTATGAAGAAATATACGTTCGCTAAGGAGGTTTTCTAATGAGCAACGCAAAAACAAAG	Amplification of the aac(6′)-Ib-cr gene
aac6-gfp-R	AAAGTTCTTCTCCTTTACTCATATGTATATCTCCTTCTTATTAGGCATGACTGCGTGTTC	Amplification of the aac(6′)-Ib-cr gene
opvA-ctxm-F	TCTTATGAAGAAATATACGTTCGCTAAGGAGGTTTTCTAATGGTTAAAAAATCACTGCG	Amplification of the bla_CTX-M-15 gene
ctxm-gfp-R	AAAGTTCTTCTCCTTTACTCATATGTATATCTCCTTCTTATTACAAACCGTCGGTGACGA	Amplification of the bla_CTX-M-15 gene
PopvAB-aac3-F	ATCTTATGAAGAAATATACGTTCGCTAAGGAGGTTTTCTAATGCATACGCGGAAGGCAATAAC	Amplification of the aac3-Ib gene
GFP-aac3-R	GAAAAGTTCTTCTCCTTTACTCATATGTATATCTCCTTCTTACTAACCGGAAGGCTCGCAAG	Amplification of the aac3-Ib gene
opvAB-ampsacB-F	TCTTATGTGTGGGTTTTATCTTATGAAGAAATATACGTTCGCTAAGGAGGTTTTCTAATGTAGGCTGGAGCTGCTTC	Amplification of the bla-sacB cassette
ampsacB-gfp-R	AAAGTTCTTCTCCTTTACTCATATGTATATCTCCTTCTTACATATGAATATCCTCCTTAGTTCC	Amplification of the bla-sacB cassette