Figure 4.

Binary properties of salt bridge of hsMDH (pink) relative to ecMDH (blue). Binary items of isolated (iso; a, f, k, p) vs networked (net; b, g, l, q) and core (c, h, m, r) vs surface (sur; d, i, n, s) are separately plotted along with total frequency (e, j, o, t). As the residues are unequal in these two proteins, normalization was done using earlier scale [40]. HB hydrogen bonded; nHB non-hydrogen bonded; L local; nL non-local; SS salt bridge in secondary structure (Helix/strand); BM bond multiplicity