Fig. 1.

Characterisation of ScFv and ScFv/RVG conjugate. The plant-produced ScFv was purified by Ni affinity chromatography. The ScFv was chemically conjugated to chemically synthesized RVG peptide to produce ScFv/RVG. ScFv and ScFv/RVG conjugate were analysed by SDS-PAGE under reducing conditions, followed by (A) staining with Coomassie blue or (B) blotting onto nitrocellulose and probing with a mouse anti-E tag antiserum. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)