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. 2019 Aug 2;37(33):4673–4680. doi: 10.1016/j.vaccine.2018.02.057

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Inhibition of entry of ScFv/RVG conjugate into nAchR-overexpressing 293 cells and neuroscreen cells by irradiated rabies virus and α-bungarotoxin. Flow cytometry on nAchR-overexpressing 293 cells pre-treated with irradiated rabies virus (A, B) and α-bungarotoxin (C, D) before incubation with ScFv (A and C), and ScFv/RVG conjugate (B and D). Flow cytometry on neuronal 2a cells pre-treated with irradiated rabies virus (E, F) and α-bungarotoxin (G, H) before incubation with ScFv (E and G) and ScFv/RVG conjugate (F and H). Solid line: no inhibitor, Dotted line: pre-treated with irradiated rabies virus or α-bungarotoxin; A representative result from triplicate experiments is shown.