Figure 5.

Effect of hypoxia (see “Materials and Methods”) on the level of FAT/CD36 and FABP 1 and 6 h after the treatment in cytosolic and membrane-enriched fraction of NES2Y cells. Levels of the tested proteins were determined using western blot analysis employing relevant antibodies (see “Materials and Methods”). Equal protein load was checked after blotting by staining with Ponceau S solution. The level of EGFR (membrane protein) and MEK1/2 (cytosolic protein) were used to confirm that the preparation of fractions was successful. The data shown were obtained in one representative experiment from at least three independent experiments.