Table 2.
Primers/probes used for site-directed mutations and the EMSA and ChIP assays.
| Primers Used for Site-Directed Mutations | |
| Name | Sequence 5′→3′ |
| mSp1-d-S | aagcggaccacaggTATgggtttccctcgagaggc |
| mSp1-d-AS | gcctctcgagggaaacccATAcctgtggtccgctt |
| mSp1-p-S | tcgggagcggaagtggTTAagtcagagcacatcc |
| mSp1-p-AS | ggatgtgctctgactTAAccacttccgctcccga |
| mNFY-p-S | gtgcggccaacggcTTTtgaggactggtcttaag |
| mNFY-p-AS | cttaagaccagtcctcaAAAgccgttggccgcac |
| mNFkB-S | accacagggcggggtCATcctcgagaggcgaacg |
| mNFkB-AS | cgttcgcctctcgaggATGaccccgccctgtggt |
| mElk-1-S | cgagtcagagcacatAAgTtgttagaagcgctggt |
| mElk-1-AS | accagcgcttctaacaAcTTatgtgctctgactcg |
| mETS1-S | gtaaaagatcgggagcTAaagtgggcgagtcagag |
| mETS1-AS | ctctgactcgcccacttTAgctcccgatcttttac |
| Probes Used in EMSA | |
| Name | Sequence 5′ to 3′ |
| Sp1-1-S | gcggaccacagggcggggtttccctcgagaggc |
| Sp1-1-AS | gcctctcgagggaaaccccgccctgtggtccgc |
| Sp1-2-S | cgggagcggaagtgggcgagtcagagcacatccg |
| Sp1-2-AS | cggatgtgctctgactcgcccacttccgctcccg |
| Note: Mutant primers mentioned above are used as probes for respective sites | |
| ChIP Assay Primers | |
| G-TMBIM6 S | cgacaagctcaggcctctgaac |
| G-TMBIM6 AS | taccagcgcttctaacaccgga |
Indication: m—mutation; S—sense; AS—antisense; G—genome.