Table 1.
Mitochondrial respiration was measured in permeabilized cardiac fibers freshly isolated at the end of reperfusion.
| Groups | Basal Respiration (nmol O2/min/mg) | ADP-Stimulated (nmol O2/min/mg) | Respiratory Control Ratio (RCR) |
|---|---|---|---|
| WT Aerobic | 0.64 ± 0.12 | 3.36 ± 0.81 | 5.26 ± 0.49 |
| sEH null Aerobic | 0.58 ± 0.15 | 2.70 ± 0.71 | 4.90 ± 0.75 |
| WT IR | 0.75 ± 0.18 | 1.02 ± 0.21 | 1.43 ± 0.11 *☨ |
| sEH null IR | 0.97 ± 0.36 | 3.95 ± 1.37 | 4.03 ± 0.31 # |
| t-AUCB IR | 0.88 ± 0.18 | 2.92 ± 0.49 | 3.87 ± 0.59 # |
Oxygen consumption was assessed using a Clark electrode connected to an Oxygraph Plus recorder where malate and glutamate were used to stimulate basal respiration. Rates were presented as Respiratory Control Ratio (RCR), which is a ratio of adenosine diphosphate (ADP)-stimulated to basal respiration. Values represent mean ± SEM, * p < 0.05 vs. WT Aerobic CT, ☨ p < 0.05 vs. sEH null Aerobic, # p < 0.05 vs. WT IR (n = 4–8 per group).