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. 2019 Jul 19;20(14):3543. doi: 10.3390/ijms20143543

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ZRSR1 expression in HEK293 murine cell lines and in mouse hypothalami. Numbers of differentially expressed genes (DEG) and differentially expressed isoforms (DEI). Significantly enriched Gene Ontology (GO) terms (p < 0.05) in DEGs. (A) Western blot analysis of ZRSR1 protein expression. Lanes 1 and 3 correspond to the murine cell lines expressing mZrsr1 and mZrsr1 truncated protein, respectively. Expression of ZRSR1 in wild-type (WT) mouse hypothalamus and Zrsr1^mu mouse hypothalamus is shown in lanes 2 and 4. Actin was used as the loading control (lower panel). (B) Venn diagram showing the correspondence between up- and down-regulated genes and isoforms in the comparison between Zrsr1^mu and WT mouse hypothalami. (C) Significant GO terms in DEGs in the pairwise comparison.