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Densitometric analysis of the Coomassie blue-stained SDS-PAGE bands. Proteins from particles incubated at 10, 20 and 30 min at 37 °C were eluted sequentially with buffers at different pH. Top panel: Proteins eluted after incubation in buffer 50 mM HEPES 0.1% OGP, pH 7 (soft corona). Lower panel: Proteins eluted after incubation in buffer 100 mM NaAc 0.1% OGP, pH 5 (hard corona). (a and c) NP-DMSA (b and d) NP-PEG-(NH2)2 (2000).
