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Evaluation of cell viability by MTT assay. (a) Caco-2 cells. (b) RAW 264.7 cells. (c) Ferritin formation measured by ELISA in Caco-2 cells exposed to 250 µM of NP-DMSA or NP-PEG-(NH2)2(2000). (d) Evaluation of macrophages activation by measurement of nitric oxide production. Data represent means ± SD (n = 3). * Shows statistical significance compared with the control (one-way ANOVA, Bonferroni´s post-hoc test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
