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. 2019 Jul 17;8(7):735. doi: 10.3390/cells8070735

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Kinase activities of CKS1-bound kinases CDKs were purified on CrCKS1 beads from cells grown in the absence or presence of caffeine, zeocin and caffeine/zeocin. Kinase activities towards histone H1 as a substrate are shown. (A) Means from at least four independent experiments are presented. (B) A representative image from one experiment is shown. Upper panel: gel stained by Coomassie Brilliant Blue for loading control, bottom panel: autoradiogram; the time of sampling in hours is depicted on the top of panels.