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. 2019 Jul 10;20(14):3388. doi: 10.3390/ijms20143388

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interaction of soybean mosaic virus (SMV-P3) and the GmHRLI protein. (A) Confirmation of the interaction of P3 with candidate interactors in yeast NMY51 cells, and the interaction intensity shown by tipping different concentrations of interaction solution. Each result was representative of three biological repeats. (B) The interaction of GmHRLI and P3 shown in Nicotiana benthamiana. Transgenic Nicotiana benthamiana expressing CFP-H2B (nuclear localized histone 2B) were used for theBiFC assays. Scal bar = 10 μm. GST and Hc-Pro were used as control, respectively. Images are representative of three separate infiltrations from two independent experiments for each interaction.