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. 2019 Jul 17;11(7):994. doi: 10.3390/cancers11070994

Figure 4.

Figure 4

Figure 4

Simvastatin induces apoptosis via BID cleavage and caspase activities in Daoy cells. (A,B) Caspase activity in Daoy cells was measured by the Caspase Glow Assay kit at 48 h. The activity of caspase 8 is shown in A, and caspases 3/7 and caspase 9 are shown in B. Statistical significance is reported by unpaired t-test for caspase 8 activity and one-way ANOVA for caspase 3/7 and 9 using GraphPad Prism 7.0. The p-value is reported as **** p < 0.0001. Data are expressed as means ±  SEM, and n = 9–10. (C–E) For Western blots, Daoy, D283, and D341 cells were collected at 24 h, 48 h, and 72 h. GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase) detection of the same membrane was used as a loading control. For the continuous full length-image of Western blot signals in Figure 4C–E, please refer to Supplementary Figure S2.