Figure 6.

The combination of sunitinib and recombinant human tumor necrosis factor-related apoptosis-inducing ligand (rhTRAIL) significantly induces c-Jun N-terminal kinase (JNK) phosphorylation. (A,B) Slides cut from formalin-fixed paraffin-embedded tissues were deparaffinized in xylene and rehydrated. Tumors were stained with an (A) anti-p-JNK or (B) anti-JNK antibody at 4 °C overnight. Tumors were then incubated for 1 h at room temperature with goat anti-rabbit horseradish peroxidase (HRP) antibody, followed by 3,3′-diaminobenzidine for 10 min. Slides were counterstained with hematoxylin. Quantification of expression levels of both p-JNK and JNK in tumor tissue is shown. Mean ± Standard Deviation (SD), n = 5. * Indicates a significant difference compared to vehicle control or ** compared to either single agent group, p < 0.05.