Figure 7.

The combination of sunitinib and recombinant human tumor necrosis factor-related apoptosis-inducing ligand (rhTRAIL) significantly decreases X-linked inhibitor of apoptosis protein (XIAP) expression and increases cleaved caspase-3 levels. (A,B) Slides were stained with an (A) anti-XIAP or (B) anti-cleaved caspase-3 antibody at 4 °C overnight. Tumors were then incubated for 1 h at room temperature with goat anti-rabbit horseradish peroxidase (HRP) antibody, followed by 3,3′-diaminobenzidine for 10 min. Slides were counterstained with hematoxylin. Quantification of expression levels of both XIAP and cleaved caspase-3-positive cells in tumor tissue is shown. Mean ± Standard Deviation (SD), n = 5. * Indicates a significant difference compared to vehicle control or ** compared to either single agent group, p < 0.05.