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. 2019 Jul 19;8(7):750. doi: 10.3390/cells8070750

Figure 2.

Figure 2

Anti-ERα Abs induced tumor growth and TAM resistance in breast cancer xenografts in Severe Combined Immunodeficiency (SCID) mice. (a) The mean (± SD) of the tumor volume of one set of experiment (10 mice/group) was shown. The tumor volumes were calculated at day 0, day 7 and day 14 using the following formula: (a × b2)/2, where a and b represent the longest and shortest diameters, respectively. At day 14, TAM + IVIG vs. TAM + anti-ERα Abs: ***, p < 0.001 by Student’s t test. (b,c) Representative sections from MCF-7 tumors treated with IVIG, anti-ERα Abs, TAM + IVIG and TAM + anti-ERα Abs immunostained with anti-Ki67 (red) antibodies and counterstained with Hoechst (blue) are shown. Only nuclear staining (plus mitotic figures) was considered positive. Ki-67 was scored by the average method as reported in Methods. Results are represented as mean ± SD. Statistical analysis: *, p < 0.05 by Student’s t test. Scale bar = 50 μm.