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. 2019 Jul 4;10(7):507. doi: 10.3390/genes10070507

Table 1.

Summary of statistics of illumine transcriptome sequencing and assembly for T. pisiformis.

Sample TpM TpA TpM + TpA
clean reads 53,815,898 45,351,168 99,167,066
Total bases (Gb) 16.0 13.48 29.48
(G + C) % 49.58 49.66 49.62
Q20 of clean reads (%) 96.79 96.65 96.72
Q30 of clean reads (%) 92.26 92.04 92.15
Numbers of transcripts / / 354,182
Mean length (transcript) / / 3209
N50 length (transcript) / / 4893
Total unigene / / 68,588
Mean length (unigene) / / 789
N50 length (unigene) / / 1485

Q20, Q30: The error rates of sequencing nucleotides <1% or 0.1%; N50: the shortest sequence length at 50% of overall length of spliced transcripts or unigenes; TpM: Larval stage; TpA: Adult stage.