Figure 2.

Ddx4⁺ oogonial stem cells (OSCs). Representative image depicts the oocyte-like differentiation of OSCs (black arrows) after 21 days of culture in the presence of fibroblasts feeder layer. Although present in variable size during their differentiation, the largest cells (diameter of approximately 80–90 µm) acquired oocyte-like features, with prominent nuclei and perinuclear accumulation of organelles. By contrast, smaller cells were considered immature OSCs and might represent the oogonial stem cell reserve.